Evaluation of immunoglobulin A-capture enzyme-linked immunosorbent assay for serodiagnosis of dengue virus infection / Masaru Nawa, Chao-Ying Pan, Wu-hsiung Tsai, Chaur-don Chan, Sanae Machida, Tomohiko Takasaki, Chang-Kweng Lim, Ming-Rong Harn, Ichiro Kurane

In order to determine the usefulness of anti-dengue IgA-capture enzyme-linked immunosorbent assay (IgA-capture ELISA), specific IgA was examined in the serum samples from 87 patients with symptoms of dengue in Kaohsiung city, Taiwan. Of these 87 patients, 34 cases who were confirmed to be dengue by reverse transcription-PCR (RT-PCR), and 39 cases who were RT-PCR-negative but IgM-positive, were separately analysed. Dengue virus-specific IgA antibody was first detected on disease day 5 and continued to be detected until day 39. The results indicate that the duration of dengue virus-specific IgA is short, and suggest that the detection of specific IgA in the serum is a useful diagnostic measure, especially when only a single serum sample is available from patients.

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