Preperation of antibodies against soluble recombinant dengue E proteins fused with glutathione's transferase / (Record no. 85829)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01941nam a2200217Ia 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 240109s2006 xx 000 0 und d |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Wang, Jiali. |
| 9 (RLIN) | 9430 |
| 245 #0 - TITLE STATEMENT | |
| Title | Preperation of antibodies against soluble recombinant dengue E proteins fused with glutathione's transferase / |
| Statement of responsibility, etc. | Jiali Wang, Na Gao, Wei Chen, Dongying Li, Limei Liu, Yingjie Wan, Jing An |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Date of publication, distribution, etc. | December 2006 |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| 337 ## - MEDIA TYPE | |
| Media type term | unmediated |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| 440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE | |
| Number of part/section of a work | 30, pages 162-170 |
| Title | Dengue Bulletin |
| 9 (RLIN) | 9431 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The envelope glycoprotein (E) of dengue (DENV) viruses mediates viral attachment and entry through membrane fusion. In this study, three fragments (domain 3a, domain 12 and domain 123a) of DENV envelope protein were inserted into plasmid pGEX-6p-1 to express glutathione's transferase (CST) fusion proteins in Escherichia coli (E. coli) strain BL21. High yields of soluble recombinant proteins were obtained as follows: 20 mg/l of GST-Ela protein, 5 mg/L of GST-E12 protein, and 3 mg/t of GST-E123a protein. Although they all failed to protect vero cells from DENV infection in virus-binding blocking assay, three proteins could be recognized by mouse serum against DENV in westem blot analysis. Then, polyclonal antibodies against either GST-E12 or GST-E3a were raised in New Zealand rabbits, and used in immunofluorescence staining. The infected vero cells showed typical cytoplasmatic fluorescence near the nucleus where the virus replication took place. The study indicated that soluble GST fusion E proteins might lack some biological activities of natural dengue E protein. But polyclonal antibodies against GST-E3a and GST-E12 might be helpful tools for further study of DENV pathogenesis in vitro. |
| 521 ## - TARGET AUDIENCE NOTE | |
| Target audience note | Nursing. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Dengue virus. |
| 9 (RLIN) | 9432 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Envelope protein. |
| 9 (RLIN) | 9433 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Glutathione's transferase (GST). |
| 9 (RLIN) | 9434 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Library of Congress Classification | Manila Tytana Colleges Library | Manila Tytana Colleges Library | REFERENCE SECTION | 01/09/2024 | 01/09/2024 | 01/09/2024 | Articles |