Studies on quercus lusitanica extracts on DENV-2 replication / (Record no. 85824)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02366nam a2200229Ia 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 240109s2006 xx 000 0 und d |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Rahman, Noorsaadah Abd. |
| 9 (RLIN) | 9404 |
| 245 #0 - TITLE STATEMENT | |
| Title | Studies on quercus lusitanica extracts on DENV-2 replication / |
| Statement of responsibility, etc. | Noorsaadah Abd. Rahman, Hadinur, Sylvia Muliawan, Nurshamimi Nor Rashid, Mudiana Muhamad, Rohana Yusol |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Date of publication, distribution, etc. | December 2006 |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| 337 ## - MEDIA TYPE | |
| Media type term | unmediated |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| 440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE | |
| Number of part/section of a work | 30, pages 260-269 |
| Title | Dengue Bulletin |
| 9 (RLIN) | 9405 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This study aimed to search for compounds with potential inhibitory activities towels DENV-2 replication In vitro inhibitory activities of plant extracts towards DENV-2 replication were studied and the proteomics profile of cells infected with dengue virus, followed by treatment with plant extracts, were mapped out. Methanol crude and fractionated extracts of Quercus lusitanica were tested. The cytotoxicity of these plant extracts was evaluated by determining the maximum non-toxic dose (MNTD) on C6/36 cells. Antiviral activity was estimated by the reduction of the cytopathic effect (CPE) of DENV 2 in C6/36 cells and by the reduction of virus titre. The crude methanol extracts of Q lusitanica at the concentration of 180 pg/ml was found to completely inhibit the dengue virus infection at TCID of 1-1000 by the absence of CPE. Protease inhibition assay of the crude and fractionated methanol extracts indicated more than 90% inhibition at the concentration of 0.2 mg/ml of the extracts. Methyl gallate purified from fractionated crude extracts of Q. lusitanica at the MNTD of 100 µg/mL showed a 96% inhibition at TCID, of 1000. DENV-2 virus protease inhibition assay of methyl gallate showed more than 98% inhibition at 0.3 mg/ml. Two-dimensional electrophoresis gels of normal, infected and treated cells showed that the treatment with crude methanol extracts as well as methyl gallate purified from the extract down-regulated the expression of the NS1 protein. |
| 521 ## - TARGET AUDIENCE NOTE | |
| Target audience note | Nursing. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Dengue virus 2 (DENV-2). replication. |
| 9 (RLIN) | 9406 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Maximiun non-toxic dose. |
| 9 (RLIN) | 9407 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Protease inhibitation assay. |
| 9 (RLIN) | 9408 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Quercus lusitanica. |
| 9 (RLIN) | 9409 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Library of Congress Classification | Manila Tytana Colleges Library | Manila Tytana Colleges Library | REFERENCE SECTION | 01/09/2024 | 01/09/2024 | 01/09/2024 | Articles |