000			02318nam a2200217Ia 4500
008			230425s2008 xx 000 0 und d
040			_cManila Tytana Colleges
100			_aAlcausin, Maria Melanie Liberty B. _93818
245		0	_aGenetic polymorphisms of glutathione-S-transferase P1, T1 and M1 in pediatric patients with acute lymphocytic leukemia in a Philippine tertiary hospital / _cMaria Melanie Liberty B. Alcausin, Pamela D. Fajardo, Catherine Lynn T. Silao, Amy Goleta-Dy, Eufrosina A. Melendres, Eva Maria C. Cutiongco-dela Paz, Carmencita David-Padilla
260			_c2008
336			_atext
337			_aunmediated
338			_avolume
440			_n42 : 2, pages 22-26 _aActa Medica Philippina _93819
520			_aIntroduction. Glutathione S-transferases (GSTs) are major detoxifying enzymes that modify susceptibility in cancers including acute lymphocytic leukemia (ALL). This paper determines the frequency of GST polymorphisms (M1, T1, P1) in Filipino ALL patients and control subjects and compares the frequencies between the two groups. Methods. Pediatric ALL patients at the UP-PGH Medical Center seen from January to June 2007 were enrolled. Age and sex matched subjects without ALL from the UP-PGH Outpatient Department were included as controls. Genomic DNA was extracted from peripheral blood of each subject. GSTM1 and T1 polymorphisms were determined using polymerase chain reaction (PCR) while restriction fragment length polymorphism (RFLP) analysis was employed for the determination of GSTP1 polymorphisms. Matched Odds Ratio was used to compare the genomic frequencies of control and ALL patients. Results. The presence of GSTT1 and GSTM1 polymorphisms showed a trend towards protection from having ALL, with OR 0.59 (95% CI: 0.24- 1.36) and OR 0.86 (95% CI: 0.36-2.00), respectively. Having the GSTP1 polymorphism was shown to be a risk factor [OR 1.7 (95% CI: 0.74- 4.15)]. Conclusion. Differences in GST polymorphism frequencies were noted between the control group and ALL patients. GSTT1 and GSTM1 polymorphisms appear protective while having the GSTP1 polymorphism confers increased risk for ALL.
521			_aNursing.
650			_aAcute lymphocytic leukemia. _93820
650			_aGlutathione-S-transferase. _93821
650			_aPolymorphic enzyme. _93822
942			_cA
999			_c84373 _d84373